Journal: PLoS ONE

Article Title: Berberine Induces Caspase-Independent Cell Death in Colon Tumor Cells through Activation of Apoptosis-Inducing Factor

doi: 10.1371/journal.pone.0036418

Figure Lengend Snippet: Cells were treated with berberine at indicated concentrations for 18 h (A and B), as described in . Intracellular ROS levels were detected using DHE probe and the amount of fluorescence was measured using flow cytometry. The fold change of the amount of fluorescence was calculated by comparing that in the treated groups to the control group (A). Cells were treated with the JC-1 probe and then analyzed by flow cytometry to measures the fraction of cells with polarized or depolarized mitochondrial membrane (B). Cells were treated with berberine at 50 µM for 18 h in the presence or absence of 5 µM of Tiron, a ROS scavenger. Cell number change (C) and LDH release (D) were detected as described in . * p <0.01 compared to the control group, # p <0.01 compared to the berberine treated group.

Article Snippet: Δ ψ was measured using the Flow Cytometry Mitochondrial Membrane Potential Detection kit (BD™ Mitoscreen) according to the manufacturer’s instructions.

Techniques: Fluorescence, Flow Cytometry